Lab 10: Proteomics 2
Uniprot: http://www.uniprot.org/
Interpro: http://www.ebi.ac.uk/interpro/
Expasy: http://us.expasy.org/
NextPROT: https://www.nextprot.org/
Questions you could ask?
Where does trypsin cut your protein? How many peptides are formed when you cut with trypsin?
What is it's molecular weight?
What PTMs are found in your protein?
*Post Translational Modifications
*Students in the past have used NetPhos or now PhosphoSitePlus to find putative phosphorylation sites in their protein.
--Paste in your protein FASTA and click all 3 sites. Check bottom of results page for image of where all of your sites are located.
SILAC |
iTRAQ |
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