Lab 7: Proteomics I
Questions you could ask using Expasy?
Where does trypsin cut your protein? How many peptides are formed when you cut with trypsin?
What is it's molecular weight?
Does my protein have a transmembrane domain?
*Post Translational Modifications
Predicting Subcellular Location
Where does your protein localize in the cell? Where do the interacting proteins locate?
(these sites use different algorithms, so check out if they give the same result)