Lab 10: Proteomics 2
Uniprot: http://www.uniprot.org/
Expasy: http://us.expasy.org/. (cool new interface in 2021)
Interpro: http://www.ebi.ac.uk/interpro/
Questions you could ask using Expasy?
Where does trypsin cut your protein? How many peptides are formed when you cut with trypsin?
What is it's molecular weight?
Does my protein have a transmembrane domain?
*Post Translational Modifications
Does your protein have any post-translational modifications (PTMs)?
*Students in the past have used NetPhos to find putative phosphorylation sites in their protein
SILAC |
iTRAQ |
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